Must the previous 214(b) refusal be disclosed on a new DS-160?

Yes. Questions about previous US visa refusals must be answered truthfully, with an accurate explanation where requested. Concealing the earlier refusal does not improve the application and may create a separate issue concerning the reliability of the information supplied. Use the old form only as a reference, checking employment, addresses, education, travel plans, and funding again. If an earlier answer was wrong, provide the correct information rather than repeat a known mistake for apparent consistency. Applicants should review the contents even when someone else prepares the form. Potentially material misrepresentation or other legal complications may require advice from a properly qualified US legal professional.