Can the previous application fee be used for a new application?

No. A new visa application after a 214(b) refusal requires a new application fee. The fee paid for the refused application is not refunded because of the refusal and does not replace the payment required for the next application. Check the Department of State and the relevant post's official instructions for current amounts, payment methods, and any category-specific fee rules. Another applicant's experience in a different country may not reflect the procedure that applies to you. Budget separately for document preparation and any necessary travel. Paying the fee does not establish substantive eligibility or determine whether a visa will be issued.